Journal: bioRxiv

Article Title: Coordinated inhibition of SOX9 and cell cycle progression by microRNA-200 restricts sebaceous gland fate specification

doi: 10.64898/2026.01.09.698672

Figure Lengend Snippet: a. Conserved miR-200 targeting sequence on the 3’UTR sequences of Csnk1a1 and Btrc . b & c. Luciferase reporter assay for Csnk1a1 (b) & Btrc (c) showing the functional relevance of miR-200 binding sites to their respective 3’UTR. A Student’s t-test was performed for statistical analysis. d. Spatial transcriptomics clusters of control and induced skin at P3. e. Schematics of scHolography analysis from ST and scRNA-seq together. f. Expression of selected marker genes along the epidermal-to-matrix axis. The solid line connects the median gene expression level at each computed distance bin along the epidermal-to-matrix axis. g-i. Expression of Wnt10a (g), Wnt10b (h) , and Sox9 (i) along the epidermal-to-matrix axis shows upregulation of Wnt10a and Wnt10b , as well as downregulation of Sox9 in the upper HF region. Solid lines connect the mean expression level for individual genes for each sample across the spatial bins. Wilcoxon test was performed for statistical analysis. j. Representative contour plot for HF-keratinocytes marked by EpCAM-APC + /SOX9-eGFP + from 2 control and 3 induced skin samples harvested at P1. k. Modal distribution of GFP intensity of the EpCAM + /SOX9-eGFP + of both control (red) and induced (blue) cells. l. SOX9 IF shows uHF-specific downregulation in induced HF compared to control. Scale bar: 20 μm. m. NFATc1 and PPARγ downregulation in induced HF reveals compromised SOX9 function. Scale bar: 20 μm. n. In SOX9 cKO and miR-200 induced samples, LEF1+ cells were detected in the uHF region marked by a white bracket whereas these LEF1+ cells were absent in the control uHF. White brackets annotate the uHF region. Scale bar: 20 μm.

Article Snippet: SOX9 IRES-eGFP (Tg(Sox9–EGFP)EB209Gsat) mouse was obtained from MMRRC (stock number: 011019-UCD) and was bred with Krt14-rtTA/pTRE2-miR-200b/a/429 mouse.

Techniques: Sequencing, Luciferase, Reporter Assay, Functional Assay, Binding Assay, Control, Expressing, Marker, Gene Expression

Journal: bioRxiv

Article Title: Coordinated inhibition of SOX9 and cell cycle progression by microRNA-200 restricts sebaceous gland fate specification

doi: 10.64898/2026.01.09.698672

Figure Lengend Snippet: a. GeneTrajectory analysis of uHF and SG cells from control reveals a distinct trajectory from the uHF progenitors driving the SG fate. b . GO analysis of 632 genes from the SG trajectory. The top enriched terms are shown. c. Quantitative analysis of the peaks identified by SOX9 Cut&Run. d. The most highly enriched motifs (HOMER de novo motif search) in 3,272 SOX9 Cut&Run peaks. e. Intersection of genes identified in the SG trajectory with genes associated with SOX-motif containing peaks (left) and GO-term analysis of these SOX9-regulated SG trajectory genes (right). f & g. IGV tracks of representative genes Cers4 (f) Sqle (g, left) and Srebf2 (g, right), showing strong SOX9 Cut&Run signals on the promoter regions of these lipid metabolism genes. Purple rectangle annotates the peaks containing SOX motifs, a black horizontal line below the SOX9 Cut&Run track marks the actual peak with red vertical line pointing to the canonical ACAAAG motif for each peak.

Article Snippet: SOX9 IRES-eGFP (Tg(Sox9–EGFP)EB209Gsat) mouse was obtained from MMRRC (stock number: 011019-UCD) and was bred with Krt14-rtTA/pTRE2-miR-200b/a/429 mouse.

Techniques: Control

Journal: bioRxiv

Article Title: Coordinated inhibition of SOX9 and cell cycle progression by microRNA-200 restricts sebaceous gland fate specification

doi: 10.64898/2026.01.09.698672

Figure Lengend Snippet: a. GO-terms of the downregulated genes in uHF progenitors upon miR-200 induction are enriched for genes associated with cell cycle and lipid metabolism. b. 136 of the CLEAR-CLIP identified miR-200 targets are downregulated in the uHF progenitors, as shown by the Venn diagram. Selected cell cycle genes that are miR-200 targets are listed. c. GO term analysis of miR-200 target genes that are downregulated in the uHF progenitors. d. Intersection of the SG trajectory genes with downregulated genes in uHF progenitors reveals the enrichment for SOX9 targets. e. Schematics of miR-200 mediated inhibition of sebaceous gland specification.

Article Snippet: SOX9 IRES-eGFP (Tg(Sox9–EGFP)EB209Gsat) mouse was obtained from MMRRC (stock number: 011019-UCD) and was bred with Krt14-rtTA/pTRE2-miR-200b/a/429 mouse.

Techniques: Inhibition